Expression and functional characterization of INPP4B in gallbladder cancer patients and gallbladder cancer cells

Abstract Background Inositol polyphosphate 4-phosphatase type II (INPP4B) is a negative regulator of the PI3K-Akt signalling pathway and plays contradictory role in different types cancers. However, its biological played by INPP4B human gallbladder cancer (GBC) has not been elucidated. In this study, we investigated expression, clinical significance function GBC patients cell lines. Methods The protein expression levels tissues normal were detected immunohistochemistry, was analysed. Knockdown overexpression GBC-SD SGC-996 cells followed proliferation, clonogenic, apoptosis detection, scratch wound-healing transwell assays used to identify vitro. Results up-regulated compared with related histopathological differentiation ( p = 0.026). Here, observed that highly expressed high-moderately differentiated tumours low-undifferentiated 0.022). Additionally, found associated overall survival 0.071) an independent prognostic factor. Furthermore, when stratified relationship between prognosis based on differentiation, progression depending degree differentiation. addition, knockdown inhibited colony formation, migration invasion cells, while had opposite effects vitro, which indicates as oncoprotein. Conclusions These findings suggested may play dual might act oncogene cells.